moses.koobotse

A highly motivated junior faculty and early-career researcher at the University of Botswana, with expertise in Cellular Pathology and Cancer Biology. My academic training and predoctoral research provided me with an excellent background in several disciplines of cancer research and diagnosis, including cytopathology, histopathology, molecular pathology and cancer biology. As a master’s degree student, I undertook research on the role of IGFBP2 and EGFR on triple negative breast cancer. Data from my dissertation contributed to a publication in a major journal of clinical pathology, which I co-authored (PMID: 24515759). As a predoctoral student, my research focused on elucidating the interplay between BRCA1 and IGF-I actions in lipid metabolism in breast cancer. I developed technical experience and expertise in cell and tissue culture methods, PCR, immunoblotting, immunofluorescence, ELISA, immunohistochemistry and subcellular fractionation among others. I have experience with analyzing subcellular contents including DNA, proteins, specific nuclear and cytoplasmic fractions, as well as protein-protein interactions. A first author publication from this work demonstrated a novel role of BRCA1 in breast cancer metabolism beyond the widely known BRCA1 function in maintaining genome integrity (PMID: 30323899). Recently, we have shown the impact of glucose on the BRCA1-mediated regulation of lipogenesis in breast cancer (PMID: 33212987).